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Alumni & Friends
Professor of Biological Sciences
We are interested in the control of transcription of
and in particular the basic molecular mechanisms governing the
interaction of transcription regulatory factors with each other and
with specific DNA sequences. Specific projects include: (1) analysis of
5S rRNA gene transcription by RNA polymerase III, (2) understanding
specific DNA (and RNA) sequence recognition by zinc finger proteins,
particularly the archetypal zinc finger protein TFIIIA, (3) applying
novel approaches to the study of the mechanism of transcriptional
activation, and (4) defining the mechanism of transcription termination
using a unique kinetic approach. The model systems we study include the
frog Xenopus laevis and the yeasts Saccharomyces cerevisiae (budding
yeast) and Schizosaccharomyes pombe (fission yeast). We also make use
of cultured eukaryotic cell lines.
C., Corey, D.A., and Setzer, D.R. Determinants of structural
stability and function in a single zinc finger: analysis of
alanine-substitution mutations in zinc finger 8 of Xenopus laevis
TFIIIA. In preparation.
Setzer, D.R., Schulman, D.B., Gunther, C.V., and Bumbulis, M.J. 2008. Use of a reporter gene assay in yeast for genetic analysis of DNA-protein interactions. Methods Mol. Biol., in press.
Schmidt, F.J. and Setzer, D.R. 2006. RNA: Transcription and RNA processing. In Biochemistry, 6th ed., T.M. Devlin, Editor. Wiley-Liss, 175-200.
Brady, K.L. and Setzer, D.R. 2005. Is there a dynamic DNA-protein interface in the TFIIIA-5S rRNA gene complex? J. Biol. Chem. 280, 16115-16124.
Brady, K.L., Ponnampalam, S.N., Bumbulis, M.J., and Setzer, D.R. 2005. Mutations in TFIIIA that increase stability of the TFIIIA-5S rRNA gene complex: unusual effects on the kinetics of complex assembly and dissociation. J. Biol. Chem., 280: 26743-26750.
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