Sequence and structural features of the NPH3/RPT2 family

Accession information for each NPH3/RPT2 family member

(simply click on GenBank or MATDB number to get more information)

Protein ID/Name

GenBank Accession #

MATDB Entry #

At1g03010

AC006550

At1g03010

At2g47860

AC005309

At2g47860

At3g49970

AL132978

At3g49970

RPT2

AF181683

At3g08570

AC012562

At3g08570

At3g08660

AC012562

At3g08660

At5g48800

AB012242

At5g48800

At5g66560

AB013389

At5g66560

At3g50840

AL049862

At3g50840

At1g50280

AC007980

At1g50280

At3g19850

AB025631

At3g19850

At3g22104/22105

AP001306; AB028622

At3g22104; At3g22105

At3g03510

AC009895

At3g03510

At5g17580

AL391151

At5g17580

At3g49900

AL132965

At3g49900

At5g48130

AB023039

At5g48130

At1g30440

AC009917

At1g30440

At5g03250

AL163002

At5g03250

At5g13600

AB006704

At5g13600

At3g44820

AL391254

At5g44820

At1g52770

AC019018

At1g52770

At3g15570

AB028619

At3g15570

NPH3

AF180390

At5g10250

AL360334

At5g10250

At1g67900

AC012563

At1g67900

At3g26490

AP001298

At3g26490

At5g47800

AB016886

At5g47800

At2g14820

AC004705

At2g14820

At2g23050

AC004401

At2g23050

At4g37590

AL035605

At4g37590

At5g67440

AB007645

At5g67440

At4g31820

AL049607

At4g31820

Because of its size, Figure 1 is only given as a PDF file - click here. You'll need Adobe Acrobat Reader to open the file.

Fig. 1. Alignment of the NPH3/RPT2 protein family.

Protein names are given to the right of the sequences, while amino acid positions are given to the left. Lines above sequences indicate regions of conserved sequence: DIa (light red), DIb (dark red), DII (blue), DIII (green), and DIV (yellow). Boxes around sequence denote the positions (NH2 and COOH boundries are "majority", boundries for any given member varies slightly) of the BTB/POZ domain (red box) and coiled-coil (violet box). Amino acid identities are shaded.

Fig. 2. "Consensus" features of the NPH3/RPT2 protein family.

Top, positions of conserved (black boxes, DIa-DIV) and variable (grey boxes) sequence. Middle, position of mitifs identified by homology to known protein-protein interaction domains. Bottom, positions of strongly hydrophobic (black boxes) and predicted surface exposed (grey boxes) regions.

Because of its size, Figure 3 is only given as a PDF file - click here. You'll need Adobe Acrobat Reader to open the file.

Fig. 3. Phylogeny of the NPH3/RPT2 family in Arabidopsis inferred from aligned cDNA sequence (1044 of 2676 aligned nucleotide sites were analyzed; 3rd codon positions were weighted 0.5 and non-conserved regions were excluded).

Heuristic parimony analyses (1000 replicates of random taxon addition using PAUP*; Swoffod, 1998) yielded a single tree of 6529.5 steps; CI=0.29 excluding uninformative characters, RI=0.37, and RC=0.11. Branch lengths are proportional to inferred character changes under ACCTRAN optimization; numbers above branches are bootstrap percentages (Felsenstein, 1985) from 100 replicates. Where known (Vision et al., 2000), tandom duplications (TD) and segmental duplications (SD) are indicated between branches.