Protein	Gene	Identification	Antibody	Characteristics	References
SP200/p200		[35S]Met labeling 1D and 2D PAGE silver staining	MUD143	Immunoprecipitates with PsB from spores	1-3
SP170		[35S]Met labeling 2D PAGE		Acidic glycoprotein: pI 4.5-4.8	4
p102/p104		[35S]Met labeling		Immunoprecipitates with PsB from spores	3, 5
SP96/p112	CotA	[35S]Met labeling [32P]Pi labeling EM IF	MUD141 MUD62 mAb 83.5	On outer spore coat layer and interspore matrix Part of the PsB multiprotein complex Modified by O-linked fucose and phosphodiester-linked N acetylglucosamine pI 4.5-4.8 Essential for spore coat viability	5-12
SP97		[35S]Met labeling 2D PAGE		Acidic glycoprotein: pI 4.5-4.8 Migrates with SP90 on 1D PAGE at 94kD	4
SP90		[35S]Met labeling 2D PAGE		Acidic glycoprotein: pI 4.5-4.8. Migrates with SP97 on 1D PAGE at 94 kD	4
SP87		Western blots EM IF PL3	Anti-PL3	Cross linked to the middle spore coat layer	13
PsB/SP85	pspB	[35S]Met labeling EM IF	MUD102 MUD50 mAb 16.1	On inner spore coat layer Part of the PsB multiprotein complex Has in vitro cellulose binding activity	3, 5, 7, 9, 14
SP82		[35S]Met labeling 2D PAGE		Acidic glycoprotein: pI 4.5-4.8	4
SP80			mAb 5.1		15
SP76		[35S]Met labeling 2D PAGE		Acidic glycoprotein: pI 5.9	4
SP75	DP87	[35S]Met labeling [32P]Pi labeling EM	MUD62 mAb 83.5	On outer spore coat layer Modified by N- glycosylation and O fucose Crosslinked into coat Immunoprecipitates with PsB from spores	5-7, 16, 17
SP72		[35S]Met labeling 2D PAGE		Acidic protein: pI 4.5-4.8	4
SP70/p78	CotB	[35S]Met labeling EM	2H3	On outer spore coat layer Modified by O-fucose Part of the PsB multiprotein complex Essential for spore coat viability	3, 6, 10, 17, 18
SP68		Coomassie Blue [35S]Met labeling 1D PAGE		Minor coat protein	1, 19
p63		[35S]Met labeling 1D PAGE		Immunoprecipitates with PsB from spores	3, 5
SP60/p58	CotC	Coomassie Blue [35S]Met labeling 2D PAGE		On inner spore coat layer Part of the PsB multiprotein complex	1, 3, 19
SP55		[35S]Met labeling 2D PAGE		Acidic protein: pI 5.7	4
p45		[35S]Met labeling 1D PAGE		Immunoprecipitates with PsB from spores	3
SP39		[35S]Met labeling 2D PAGE		Minor coat protein	4
SP33		[35S]Met labeling 2D PAGE		Basic protein: pI 6.6-7.5	4
SpiA	Dd31	Western blots SpiA	anti SpiA	On inner spore coat layer Essential for spore coat viability	20, 21
SP3		[35S]Met labeling 2D PAGE		Minor coat protein	4

REFERENCES

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12 Srinivasan, S. et al. (1995) unpublished.
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21 Richardson, D.L. et al. (1991) A prespore gene, Dd31, expressed during culmination of Dictyostelium discoideum. Dev. Biol. 144, 269-280